수종의 성장인자가 초기 골치유과정에 미치는 영향에 관한 연구
CHANGES OF CYCLINS, CYCLIN DEPENDENT KINASES, CYCLIN DEPENDENT KINASE INHIBITORS DURING GLOSSAL DEVELOPMENT IN THE RATS
전윤옥, 이상철,
소속 상세정보
전윤옥 ( ) - 경희대학교
이상철 ( ) - 경희대학교 치과대학 구강악안면외과학
KMID : 0355619970230040581
Abstract
結論
家 의 大腿部에 IGF-I, PDGF, TGF-β, 그리고 EGF를 titanium 및 象牙質에 塗布하여
埋植한후 1日, 3日, 그리고 7日재에 犧牲시켜 走査電子顯微鏡으로 觀察한 結果 다음과 같은
結論을 얻었다.
1. 埋植 1日 後의 骨髓細胞의 附着細胞數는 對照群에 比하여 모든 成長因子에서 有意性
있게 增加하였지만, 이중 IGF-I, PDGF의 境遇 顯著히 增加하였고, 다음으로 EGF, TGF-β
순이었다.
2. 埋植 3日 後의 附着細胞數는 모든 成長因子에서 類似한 附着細胞數를 보였다.
3. 埋植 3日의 骨髓細胞의 附着形態는 IGF-I와 PDGF가 塗布된 埋植體의 表面이 가장 넓
고 납작하게 附着된 骨髓細胞들이 觀察되었으나, EGF는 中等度, TGF-β는 對照群과 함께
微弱한 細胞附着形態를 나타내었다.
4. 埋植 7日 後의 骨形成 度는 對照群에 比해 實驗群에서 顯著하였으며, PDGF와 TGF-
β 投與群에서 旺盛한 骨成長을 보였으며, IGF-I 塗布群에서는 新生骨이 양쪽 骨端쪽보다는
콜라겐쪽으로 側面에서 成長된 것이 觀察되었고, EGF에서는 新生骨이 거의 觀察되지 않았
다.
이상의 結果로 骨組織 移植, 人工齒牙 埋植時 成長因子를 利用함으로서 初期 治癒過程의
安定性에 寄與하여 骨組織의 移植 및 思料되나 앞으로도 더 많은 硏究와 觀察이 必要할 것
으로 思料된다.
#초록#
The molecular mechanisms that regulate glossal muscle cell cycle and terminal
differentiation remain largely unknown. To determine which cyclins, cyclin dependent
kinases (CDKs), cyclin dependent kinase inhibitors (CKIs) are important for glossal cell
proliferation, we have examined expression of cyclins CDKs, CKIs during normal glossal
muscle development in the rat.
All cyclins, CDKs, and KIP/CIP family of CKIs were highly expressed during fetal
glossal muscle development, then they decreased at different rates after birth. While the
mRNAs of cyclin Dl, D3, E , A, and B decreased gradually in glossal muscle during all
stages of development, the protein levels of these cyclins decreased differently in tongue
during pre- and postnatal development. While the functionally active formed of cyclin
Dl, cyclin D3 and E proteins were observed until 7 days after birth, cyclin A and B
proteins were decreased more slowly. While the CDK4, CDK6, CDK2, cdc2, and
proliferating cell nuclear antigen (PCNA) proteins were higllly present during fetal
glossal muscle development and gradually decreased during postnatal development.
Particularly, cdc2 levels decreased markedly after birth. Immunohistochemical data for
PCNA was consistent with Western blotting data for PCNA temporally and spatially.
The mRNA and protein levels of p21, p27, and p57 were high, then their levels changed
differently during glossal development. While the mRNA levels of p21 and p57 decreased
gradually, the mRNA level of p27 did not change during glossal development. While the
protein levels of p21 and p57 in tongue decreased markedly after birth, the protein levels
of p27 increased slightly after birth, then decreased at adulthood.
These findings suggest that the all cyclins and CDKs observed are involved in glossal
muscle cell cycle, and reduction of cyclins and CDKs and induction of p21 are
associated with the withdrawal of glossal muscle cell cycle after birth.
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